CLI arguments
epiout
epiout [OPTIONS]
Options
- --bed <bed>
Required bed file containing accessible regions
- --alignments <alignments>
Required file containing bam files for each samples. Either each line is bam file (Ex: a.bam b.bamc.bam) or first columns is sample and second column is path to bam Ex: (a a.bam b b.bam c c.bam)
- --output_prefix <output_prefix>
Required Output prefix for {prefix}.raw_counts.csv, {prefix}.counts.csv, {prefix}.bed
- --cores <cores>
Number of cores to use for paralell counting.
- --mapq <mapq>
Minimum read quality of reads.
- --min_count <min_count>
Minimum count at least one sample.
- --min_percent_sample <min_percent_sample>
minimum percentange of sample peak with at least one read.
- --subset_chrom
Subset chr1 to 22 and X,Y
- --file_format <file_format>
File format of count table
epiout
epiout [OPTIONS]
Options
- --count_table <count_table>
Required Path to count table csv containing peaks (rows) x samples (columns)
- --output_prefix <output_prefix>
Required Output prefix for {prefix}.h5ad and and {prefix}.result.csv.
- --confounders <confounders>
Known confounding factors as tsv file where each row is a sample and each column is a confounding factor.
- --bottleneck_size <bottleneck_size>
Predefined bottleneck size. If not provide, will be tuned.
- --cores <cores>
Number of cores to use for paralell counting.
epiannot
epiannot [OPTIONS]
Options
- --bed <bed>
Required bed file containing accessible regions to annotate
- --config <config>
Required config file containing bed and hic files to annotate against
- --output_prefix <output_prefix>
Required Output prefix for {prefix}.annotation.csv, {prefix}.contact.csv,`{prefix}.gtf.csv`, {prefix}.genes.csv files.
- --gtf <gtf>
GTF files to obtain genes.
- --chrom_sizes <chrom_sizes>
Chrom sizes files faidx {fasta} -i chromsizes > {chrom_sizes}
- --counts <counts>
Path count table of accessible reads.
- --gene_types <gene_types>
List of gene types separated by comma.
- --hic_bin_vicinity <hic_bin_vicinity>
Number of bin distance between peaks to consider in conduct analysis.
- --hic_normalization <hic_normalization>
Hic normalization method.
- --hic_binsize <hic_binsize>
Hic bin size.
- --hic_min_read <hic_min_read>
Minimum number of hic min reads to include
epiannot_create
epiannot_create [OPTIONS]
Options
- --tissue <tissue>
Tissue, cell line, primiary or differentialed cell
- --output_dir <output_dir>
Required Output directory of create config and download files
- --tf
If include TF-ChIP-seq marks
- --hic
If inclice Hi-C
- --ccres
If include ccres
epiannot_list
List available tissues, cell lines, primary or differentiated cells.
epiannot_list [OPTIONS]